Sequencing, Gene Expression and Genotyping Services
The Center for Genomic Sciences (CGS) Core
Facility offers a wide range of high-throughput genomic services for
high-confidence, cost-effective genotyping using different state-of-the-art
genomic platforms. CGS services include sequencing, single nucleotide
polymorphism genotyping, and gene expression profiling services to
researchers in the academic, industrial, and pharmaceutical communities.
1. Sequencing: At the CGS Core
Facility, we offer sequencing services via two different sequencing
platforms. The first one uses 454 technology and the 454 Life Sciences Genome Sequencer 20
Instrument for fast sequencing of microbial genomes. The Big Dye
technology and the ABI 3730xl
instrument constitute the second platform mainly used for human genomes
candidate gene studies and SNP discoveries or for microbial genomes gap
closures.
2. Gene Expression: The Illumina BeadArray
Technology (Illumina's BeadStation
500G System) is used for gene expression profiling. The Illumina
BeadArray GeneChip System is a commercial platform that enables rapid,
reproducible, and accurate microarray analysis on a genome-wide scale.
3. Genotyping: SNP genotyping
performed at the CGS Core Facility also exploits Illumina bead-based array
platform supported by a very reproducible and high-confidence GoldenGate®
Assay protocol. We offer very flexible options to our customers to perform
either custom-SNPs or commercially available SNPs panels from Illumina, thus
we can accommodate smallest pilot scale projects to large-scale projects,
with throughput ranging from several hundreds of SNPs to several thousand
genotypes per day.
For additional information:
Please contact Dr. Fen Hu for general
inquiries, details on the cost of our services and procedures to submit
specimens.
Fen Ze Hu, Ph.D.
Phone:
412-359-8094
Toll-Free: 888-887-7729
Fax:
412-359-4377
E-mail: CGS@wpahs.org
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